Phys. Rev. E 71, 011909 (2005) [8 pages]Nucleation and growth in one dimension. II. Application to DNA replication kineticsReceived 30 July 2004; published 21 January 2005 Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems: namely, spatial inhomogeneity, measurement noise, and finite-size effects. After evaluating how each of these affects the measurements of I(t) and v, we give guidelines for the design of experiments. These ideas are then discussed in the context of the DNA-replication experiments. © 2005 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevE.71.011909
DOI:
10.1103/PhysRevE.71.011909
PACS:
87.16.Ac, 05.40.−a, 02.50.Ey, 82.60.Nh
|
|
About | Terms and Conditions | Subscriptions | Search | Help
Use of the American Physical Society websites and journals implies that the user has read and agrees to our Terms and Conditions and any applicable Subscription Agreement. Physical Review®, Physical Review Letters®, Reviews of Modern Physics®, and Physical Review Special Topics® are trademarks of the American Physical Society.