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Phys. Rev. E 55, 3762–3764 (1997)

Crystallization of globular proteins

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W. C. K. Poon
Department of Physics and Astronomy, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JZ, United Kingdom

Received 29 July 1996; published in the issue dated March 1997

Globular proteins can be crystallized by the addition of electrolyte or nonadsorbing polymer. Too much additive, however, gives rise to amorphous precipitates. By comparing existing data on various proteins with previous work on a model colloid-polymer mixture, we suggest an explanation for noncrystallization in terms of a hidden gas-liquid binodal inside the equilibrium fluid-crystal region of the phase diagram.

© 1997 The American Physical Society

URL:
http://link.aps.org/doi/10.1103/PhysRevE.55.3762
DOI:
10.1103/PhysRevE.55.3762
PACS:
87.15.Da, 82.70.Dd